Coding
Part:BBa_K5387000:Design
Designed by: iGEM24_Linkoping Group: iGEM24_Linkoping (2024-08-20)
Design Notes
The sequence has been optimized for expression in E. coli.
The vector used was pET-28a(+), which His-ag we utilized when expressing our protein.
Primers for cloning into pET-28a(+) between restriction sites NdeI and EcoRI:
- fwd: 5'-GCGCCATATGGCAACATATAAAGTTAGGG-3'
- rev: 5'-GCGCGAATTCTTAGATGCTGATGG-3'